Louisiana Marsh Dieback - Growth Response of Brown Marsh Populations of Spartina alterniflora to Aluminum data Task 5.1


Louisiana State University, Wetland and Biogeochemistry Institute and Department of Oceanography and Coastal Studies
Publication_Date: 2005
Louisiana Marsh Dieback - Growth Response of Brown Marsh Populations of Spartina alterniflora to Aluminum data Task 5.1
Geospatial_Data_Presentation_Form: Digital tabular data
Barataria-Terrebonne National Estuary Program Scientific-Technical Committee
Publication_Date: 20010207
Requests for submission of Scope of Services for Marsh Dieback and Nutria Control Emergency Response
Geospatial_Data_Presentation_Form: document
Publication_Place: Thibodaux, LA
Barataria-Terrebonne National Estuary Program Scientific-Technical Committee
Online_Linkage: <http://www.brownmarsh.net/reports/brown_marsh_rfp.pdf>
This data set consists of experimentation on Spartina alterniflora and the effects that aluminum has on the primary coastal Louisiana plant species. Soluble aluminum occurs through the oxidation of metal sulfides in wetland soils; during drought situations, the soils may experience a higher than normal amount. The spring of 2000 was such a situation. Greenhouse experiments were conducted on six genotypes of salt marsh cord-grass, five surviving and one commerical variety.
To determine if genotyptic resistance to aluminum exist in Spartina alterniflora.
Beginning_Date: 2001
Ending_Date: 2005
Currentness_Reference: observed
Progress: Complete
Maintenance_and_Update_Frequency: None planned
Description_of_Geographic_Extent: Coastal Louisiana
West_Bounding_Coordinate: -93.910681
East_Bounding_Coordinate: -88.777417
North_Bounding_Coordinate: 30.509791
South_Bounding_Coordinate: 28.892107
Theme_Keyword_Thesaurus: None
Theme_Keyword: aluminum
Theme_Keyword: brown marsh
Theme_Keyword: salt marsh cord-grass
Theme_Keyword: genotype
Theme_Keyword: dieback
Theme_Keyword: soluble aluminum
Theme_Keyword: concentration
Theme_Keyword: saltmarsh
Theme_Keyword: wetland soil
Theme_Keyword: resistance
Theme_Keyword: stem height
Theme_Keyword: stem elongation
Theme_Keyword: toxicity
Place_Keyword_Thesaurus: None
Place_Keyword: Louisiana
Place_Keyword: Coastal Louisiana
Place_Keyword: LA
Place_Keyword: Southern Louisiana
Place_Keyword: Terrebonne Basin
Place_Keyword: Barataria Basin
Taxonomic_Keyword_Thesaurus: None
Taxonomic_Keywords: Spartina alterniflora
Taxonomic_Keywords: species
Taxon_Rank_Name: Kingdom
Taxon_Rank_Value: Plantae
Taxon_Rank_Name: Division
Taxon_Rank_Value: Magnoliophyta
Taxon_Rank_Name: Class
Taxon_Rank_Value: Liliopsida
Taxon_Rank_Name: Subclass
Taxon_Rank_Value: Commelinidae
Taxon_Rank_Name: Order
Taxon_Rank_Value: Cyperales
Taxon_Rank_Name: Family
Taxon_Rank_Value: Poaceae
Taxon_Rank_Name: Genus
Taxon_Rank_Value: Spartina
Taxon_Rank_Name: Species
Taxon_Rank_Value: alterniflora
Applicable_Common_Name: smooth cordgrass
Data generated by the Brown Marsh principal investigators will be available only to the project’s other principal investigators, following the established QA/QC review, for a period of two years. After two years from the finalization of the data, it shall be made available to the public. Please contact the principal investigator for exact date of availability. It is strongly recommended that these data be directly acquired from the Brown Marsh Database Information Management System server and not indirectly through other sources which may have changed the data in some way.
Acknowledgement of the principal investigator as a data source would be appreciated in products developed from these data, and such acknowledgement as is standard for citation and legal practices for data source is expected by users of these data. Users should be aware that comparison with other data sets for the same area from other time periods may be inaccurate due to inconsistencies resulting from changes in mapping conventions, methodologies, data collections and computer processes over time. The principal investigator and the distributor shall not be liable for improper or incorrect use of these data, based on the description of appropriate/inappropriate uses described in this metadata document. The principal investigator and distributor make no claims as to the data’s suitability for other purposes. These data are not legal documents and are not to be used as such.
Louisiana State University, Wetland Biogeochemistry Institute and Department of Oceanography and Coastal Studies
Contact_Person: Irving A. Mendelssohn
Contact_Position: Professor
Address_Type: Physical
Address: Louisiana State University
City: Baton Rouge
State_or_Province: LA
Postal_Code: 70803
Country: USA
Contact_Voice_Telephone: 225 578-6425
Contact_Facsimile_Telephone: 225 578-6423
Contact_Electronic_Mail_Address: imendel@lsu.edu
Native_Data_Set_Environment: Microsoft Excel 2000
Analytical_Tool_Description: SAS Mixed Model: used to conduct statistical analysis.
Online_Linkage: <http://www.sas.com>
Tool_Access_Instructions: See website for information
Contact_Organization: SAS
Address_Type: Mailing
Address: 100 SAS Campus Drive
City: Cary
State_or_Province: NC
Postal_Code: 27513-2414
Country: USA
Contact_Voice_Telephone: 919 677-8000
Contact_Facsimile_Telephone: 919 677-4444

The accuracy of the measured attributes is dependent on the amount of human error, which is unknown. The accuracy of the calculated attributes is dependent of the accuracy of the equations used and the statistical software. Means and standard errors were considered in the analysis portion.
All specimens were labeled and randomly assigned to control and experimental treatments for a total of 48 samples, 8 of each of the six genotype - four for the control and four for the experimental treatments. The sand media was treated to remove any matter that would bind to aluminum. All plants, controlled or experimental, were allowed to accumulate for a four week period. Nitrogren and phosphorus amounts were monitored with a reagent test kit. Bi-weekly monitoring was conducted to ensure that required pH level was maintained.
Completeness_Report: There are no known omissions in the data.
Methodology_Type: Lab
Methodology_Keyword_Thesaurus: None
Methodology_Keyword: cultivar
Methodology_Keyword: concentration
Methodology_Keyword: mM
Methodology_Keyword: ebb and flow hydroponic table
Methodology_Keyword: hyproponic solution
Methodology_Keyword: pumps
Methodology_Keyword: growth chamber
Methodology_Keyword: acid bath
Methodology_Keyword: pH
Methodology_Keyword: control unit
Methodology_Keyword: experimental unit
Methodology_Keyword: muratic acid
Methodology_Keyword: sand culture
Preparation and setup - Specimens of Spartina alteriflora were obtained from the USDA-NCRS and assigned designations. A commerically available cultivar was also obtained. The samples were separate from their existing containers and their roots washed. The wet weight was recorded. The specimens were transplanted into their allocated treatment areas. The sand media was prepared by rinsing and leaching approximately 320 lbs of Play-Sand in a trash can fitted with a bottom drain and filter. The sand was leached and rinsed for 12 hours; this procedure was conducted twice. Muratic acid was added till the sand was submerged. Acid was allowed to drain 24 hours later; this procedure was also conducted twice, but followed with three rinses and drainings. Any visible traces of calcerous particles, silts, clay and organic material was removed. The specimens were placed in one of two identical ebb-and-flow hydroponic tables (control or experimental) within a growth chamber. The plants were potted into 1 gallon-trade-gallon pots. The tables were connected to a 100L sump. A 110 volt appliance timer was used to cycle two 12 volt-10 amp power transformers on or off every 15 minutes. Two 2000 gallon per hour 12 volt-10 amp centrifugal submersible pumps were attached to the floor of each sump. The pump was connected to the flow table by a flexible 1 inch diameter hose. Rigid 2 inches PVC pipes returned the water to the sump via the wier, which was set at 17cm. The twenty-four containers, lined with hardware cloth and filled with the acid washed sand and a transplanted sample were contained within each flow table. When the pump operated, a hydroponic nutrient solution would flood the table and flow around and into the plant containers. The solution was made by adding 50% dilute Hoagland's stock solution to each 100L of tap water. Before this was done, the water was prepared by the following additions - Instant-Ocean salt until 23 ppt. The pH was also reduced below 4. After 7 days, the evaporated water was replaced, pH was measured, recorded and adjusted, salinity measured and recorded, and N and P added to replace lost nutrients. After 14 days, the solution was replaced and the Al concentration was increased in the experimental unit.
Methodology_Type: Lab
Methodology_Keyword_Thesaurus: None
Methodology_Keyword: record
Methodology_Keyword: measurement
Methodology_Keyword: stem height
Methodology_Keyword: stem elongation
Methodology_Keyword: stem count
Methodology_Keyword: necrotic tissue
Methodology_Keyword: biomass
Methodology_Keyword: root material
Methodology_Keyword: stem material
Experimentation and data collection - Data were collected at two week intervals. This was conducted the week following the nutrient solution replacement and dose increase. Stem height was determined by marking one low to medium height stem with lab tape and recording stem height initially and after a 3 day interval. Stem height within each container was measured in centimeters from the top of the container using a meter ruler. A 1cm diameter dowel was placed across the rim of the trade gallon to determine the base for the ruler. All live stems, regardless of their height were counted. The necrotic tissue was removed throughout the experiment and combined with the final biomass for final weighting. On the last day of the experiment, the root material was separated from the stem material. The separated biomass were put into an oven for 72 hours at 80C.
Methodology_Type: Lab
Methodology_Keyword_Thesaurus: None
Methodology_Keyword: SAS
Methodology_Keyword: statistical analysis
Methodology_Keyword: means
Methodology_Keyword: Saxton's macro
Methodology_Keyword: percentage
Analysis - Computed from the raw data were stem elongation rate (cm?d), cumulative stem height or the total of all the stem heights for each container (cm), and the relative growth rate (cm cm-1 day-1). Stem elongation rate was calculated for each container by first finding the difference in the stem height data and dividing by the time interval. Means and standard errors were graphed as percent of the control. The stem count and cumulative stem height data of each sample were combined by genotype and into four control and four experimental samples, then means and standard errors were graphically reproduced as both percent of the control and relative growth rate. SAS's Mixed Model was used to conduct statistic analysis. The least square means was used to compare between individual treatments when interaction was significant. A Saxton's macro for converting mean separation output to letter grouping in Proc Mixed was used. The measured variables were converted to the percentage of the control of each genotype.
Process_Description: See methodology
Process_Date: 2002-2005

All measured and calculated attributes, such as stem heights, stem counts, stem elongation, etc in this subtask were taken for the overall results, the comparison of Al toxicity for each genotype and the resistance of Spartina alterniflora to Al.
Becker, Daniel F. and Irving A. Mendelssohn, Brown Marsh Project: Task V.1 Accession Characterization Subtask: Growth Response of Brown Marsh Species Populations to Stressor Factors. 2005. Louisiana State University.

Contact_Organization: U.S. Geological Survey, National Wetlands Research Center
Contact_Person: Scott Wilson
Contact_Position: Electrical Engineer
Address_Type: Mailing and Physical
Address: 700 Cajundome Blvd
City: Lafayette
State_or_Province: LA
Postal_Code: 70506
Country: USA
Contact_Voice_Telephone: 337 266-8664
Contact_Facsimile_Telephone: 337 266-8513
Contact_Electronic_Mail_Address: scott_wilson@usgs.gov
Although these data have been processed successfully on a computer system at the designated principal investigator’s facility, no warranty expressed or implied is made regarding the accuracy or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. This disclaimer applies both to individual use of the data and aggregate use with other data. It is strongly recommended that these data are directly acquired from the official Brown Marsh Database Information Management System server or from the principal investigator, and not indirectly through other sources which may have changed the data in some way. It is also strongly recommended that careful attention be given to the contents of the metadata file associated with these data. The Brown Marsh committee shall not be held liable for improper or incorrect use of the data described and/or contained herein. So, these data are provided as is and without any express or implied warranties. Also, use of trade names or commercial products in the metadata file is solely for the purpose of providing specific information, and does not imply recommendation or endorsement. Any downloading and use of these data signifies a user’s agreement to comprehension and compliance of the disclaimer. Insure all portions of metadata are read and clearly understood before using these data in order to protect both user and principal investigators interests.
Format_Name: Microsoft Excel
Format_Version_Number: 2000+
File_Decompression_Technique: none
Network_Resource_Name: <http://www.brownmarsh.net>
Fees: None, if available online
Custom_Order_Process: None

Metadata_Date: 20051104
Contact_Organization: U.S. Geological Survey/NWRC
Contact_Position: Metadata specialist
Address_Type: Mailing and Physical
Address: 700 Cajundome Blvd
City: Lafayette
State_or_Province: LA
Postal_Code: 70506
Country: USA
Contact_Voice_Telephone: 337 266-8500
Contact_Facsimile_Telephone: 337 266-8513
Metadata_Standard_Name: Content Standard for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Biological Data Profile of Content Standard for Digital Geospatial Metadata
Online_Linkage: <http://www.fgdc.gov/standards/status/sub5_2.html>
Metadata_Access_Constraints: None
Acknowledgement of the National Wetlands Research Center as the metadata source would be appreciated. Please cite the original metadata when using portions to create another record for slightly altered data, such as reprojection.

Generated by mp version 2.7.33 on Tue Nov 08 10:52:09 2005